Developing Novel Therapeutics to Treat a Broad Array of Diseases

Exoneural Medicine Platform™ (EMP)

Our platform has been developed to generate advanced starting points for drug discovery, leveraging exoneural biology for therapeutic intervention. The platform has six core components: proprietary imaging, co-culture technology, neural cell control, neural functional genomics, neural pathway bioinformatics, and a custom neuropharmacopeia. In combination, these elements empower Cygnal Therapeutics to create drugs by rapidly identifying key neural pathways implicated in numerous disease settings.

Drug Discovery Engine

Cygnal Therapeutics has assembled an interdisciplinary team of proven drug hunters skilled in the identification and development of novel therapeutics. Our team is adept at designing and developing both small and large molecule modalities. The team's deep experience in protein engineering and medicinal chemistry, combined with a broad pre-existing neuropharmacopeia, allow for innovative and rapid precision drug design. Coupled with a rich target pipeline, Cygnal aspires to produce multiple exoneural drugs across a diverse set of disease indications.

Exoneural Medicine
Platform™ (EMP)

Drug
Discovery

Neural Pathway Bioinformatics

Cygnal is studying neural pathways in the context of human tumors and model systems. We examine both bulk tumor and single cell RNA sequencing to create novel neural signatures, including neural progenitors. We are using these signatures to probe human tumor samples and model systems to help us understand interactions between neurons and the tumor microenvironment.

Neural Functional Genomics

We use CRISPR screening technologies on a subset of the genome (the "neurome") to identify targets for drug development. Together with multiple in vitro and in vivo model systems, we uncover the role of exoneural genes in disease. In addition, our CRISPR screen data analysis identifies synthetic lethal paradigms of interest.

Neuropharmacopia

Neuroscience drug discovery efforts to date have generated hundreds of small and large molecules that we can use to interrogate neuronal protein function in the context of non-canonical disease systems. We use these tool molecules as part of our target ID, validation, and discovery efforts.

Co-Culture

We have adapted co-culture systems to study neuron-cancer and neuron-immune cell interactions. We use these systems to interrogate changes in cell growth, migration, and cytokine profiles in target cells, directing us towards new pathways and targets for drug discovery.

Neural Imaging

Cygnal uses a combination of imaging methodologies including light sheet; confocal and electron microscopy combined with CLARITY; and immunofluorescence to characterize interactions between neurons and non-neuronal cells in multiple disease states. We define and quantitate these morphological features to enable screening.

Neural Cell Control

We've developed and adapted tools from neuroscience and applied them to neurons, both in vitro and in vivo, for the manipulation of neuronal function and the study of these functions on disease models. These powerful tools, which include chemogenetics and AAV delivery, allow for key target validation and proof-of-concept studies.

PlatformEnginesDiagram_CygnalTx
EMP_PlatformDiagram-Mobile_CygnalTx
EMP-Icon_Neuropharmacopeia_CygnalTx

Neuropharmacopia

Neuroscience drug discovery efforts to date have generated hundreds of small and large molecules that we can use to interrogate neuronal protein function in the context of non-canonical disease systems. We use these tool molecules as part of our target ID, validation, and discovery efforts.

EMP-Icon_FunctionalGenomics_CygnalTx

Neural Functional Genomics

We use CRISPR screening technologies on a subset of the genome (the "neurome") to identify targets for drug development. Together with multiple in vitro and in vivo model systems, we uncover the role of exoneural genes in disease. In addition, our CRISPR screen data analysis identifies synthetic lethal paradigms of interest.

EMP-Icon_Bioinformatics_CygnalTx

Neural Pathway Bioinformatics

Cygnal is studying neural pathways in the context of human tumors and model systems. We examine both bulk tumor and single cell RNA sequencing to create novel neural signatures, including neural progenitors. We are using these signatures to probe human tumor samples and model systems to help us understand interactions between neurons and the tumor microenvironment.

EMP-Icon_NeuralCellControl_CygnalTx

Neural Cell Control

We've developed and adapted tools from neuroscience and applied them to neurons, both in vitro and in vivo, for the manipulation of neuronal function and the study of these functions on disease models. These powerful tools, which include chemogenetics and AAV delivery, allow for key target validation and proof-of-concept studies.

EMP-Icon_Imaging_CygnalTx

Neural Imaging

Cygnal uses a combination of imaging methodologies including light sheet; confocal and electron microscopy combined with CLARITY; and immunofluorescence to characterize interactions between neurons and non-neuronal cells in multiple disease states. We define and quantitate these morphological features to enable screening.

EMP-Icon_Co-culture_CygnalTx

Co-Culture

We have adapted co-culture systems to study neuron-cancer and neuron-immune cell interactions. We use these systems to interrogate changes in cell growth, migration, and cytokine profiles in target cells, directing us towards new pathways and targets for drug discovery.

Cygnal has identified multiple targets with unique exoneural function

Our novel biological insights have revealed the interplay between the peripheral nervous system and multiple disease states. We’ve discovered several classes of exoneural targets with unappreciated functions in new contexts.

Axon Guidance

These factors determine the direction and extent of axon growth towards the correct targets for innervation.

Synapse Formation

These proteins form a synapse and mediate contact between neurons and other cells.

Neurotransmission

These molecules are deployed at synapses for information transfer between nerves and peripheral tissues.

Regulation of Membrane Potential

Through this process, proteins or molecules regulate ionic balance in cells and extracellular spaces.

Cygnal has identified multiple targets with unique exoneural function.